BGB Column Selection Guide
Use this checklist before requesting a BGB analytical column quotation.
For HPLC and UHPLC
Provide separation mode, current phase, USP code, dimensions, particle size, pore size, pH range, analyte, mobile phase, temperature, flow rate and instrument pressure limit.
For GC
Provide stationary-phase chemistry, polarity, length, internal diameter, film thickness, temperature program, analytes, detector and existing part number.
For flash and preparative work
Provide sample mass, target compound, impurities, solvent system, required bed mass, pressure limits and desired scale-up path.
Send your method details
Akira will review the information and suggest a suitable product for technical assistance from Akira.
Selection workflow
Start with analyte and method requirements, then compare stationary phase, dimensions, particle or film characteristics and instrument limits. Finish by reaching Akira for the exact manufacturer part number before quotation.
Frequently Asked Questions
Technical answers for common BGB column selection, compatibility and quotation questions.
What is the first step in chromatography column selection?
Identify the separation mode, analyte properties, sample matrix, current method requirements and instrument limits before comparing phases or dimensions.
How important is an existing column part number?
It is extremely useful because it can reveal phase chemistry, dimensions, particle size and hardware configuration, reducing the risk of selecting an incorrect replacement.
Should selectivity or efficiency be prioritized?
Selectivity usually has the greatest impact on resolution. Efficiency can then be optimized through particle size, length, flow rate and system performance.
How do instrument pressure limits affect column choice?
Smaller particles and longer columns generate higher pressure. The selected configuration must remain within the instrument and column pressure ratings.
When is a guard column recommended?
It is recommended for dirty samples, complex matrices, frequent injections or methods where analytical-column replacement is costly.
Can a shorter column replace a longer one?
Sometimes, but retention, resolution and system suitability may change. Any dimensional change should be technically evaluated and validated where required.
What information should be sent for selection assistance?
Send the current column details, analytes, mobile phase, flow rate, temperature, detector, system pressure and required performance.
Does Akira provide method development services?
Akira provides column-selection and product-identification assistance. Formal method development or validation scope should be requested separately from Akira.